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Mechanism of Irbesartan suppressing oxidative stress in high glucose induced endothelial microparticles release in human umbilical vein endothelial cell.

Objectives Postprandial hyperglycemia is considered as a risk factor for cardiovascular disease. The aim of this study was to observe the potential effects and mechanism of an angiotensin II type 1 receptor (AT1) antagonist Irbesartan on the high glucose-induced release of endothelial microparticles (EMPs) in cultured human umbilical vein endothelial cells (HUVECs). Methods The cultured HUVEC was divided into four groups. There are control group (5.5 mmol/L glucose), high glucose group (33.3 mmol/L glucose), Irbesartan, and diphenyleneiodonium (DPI), an inhibitor of NADPH oxidase (NOX), treatment group. For Irbesartan and DPI treatment groups, the cells were incubated with Irbesartan (10–5 mol/L) and DPI (10 μmol/L) respectively for 1 h, and subsequently stimulated with 33.3 mmol/L high glucose for another 24 h, 48 h and 72 h respectively. EMPs level and intracellular reactive oxygen species (ROS) were detected by flow cytometry. NADPH oxidase 4 (NOX4) and endothelial nitric oxide synthase (eNOS) expressions in HUVECs was detected by RT-PCR and western blot.. ….. […]

Artículo completo en: https://www.clinicalkey.com/#!/content/journal/1-s2.0-S0167527309010717

Mechanism of Irbesartan suppressing oxidative stress in high glucose induced endothelial microparticles release in human umbilical vein endothelial cell




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